Figure 4
Dual-luciferase reporter gene assays of pGL4.11-PTGR1 promoter in the HepG2 cell line. (a) Schematic representation of pGL4.11-PTGR1 promoter plasmids. The red-dashed and gray-shaded boxes indicate ZFS and TE (MER4-int), respectively. (b) Transcription activities of ZFS, including TE-derived ZFS, in the PTGR1 promoter. pGL4.11-PTGR1 promoter plasmids were transiently transfected into HepG2 cell, and luciferase assay was performed 24 h later. The results are expressed as the ratio of the luciferase activity to that of the promoterless-pGL4.11 reporter plasmid. Relative luciferase activity was obtained by normalizing the activity of firefly luciferase with Renilla luciferase. All assays were performed in triplicates, and data were presented with error bar indicating the mean ± standard deviation (SD). Student’s t test was used to determine the significance of the results (*p < 0.05, **p < 0.01).
