Figure 5
miR-6867-5p suppresses the PTGR1 gene expression by interacting with ZFS. (a) Stem-loop structure of miR-6867 as determined by the mfold web server. The miR-6867-5p sequence and its seed region (5′-GUGUGUG-3′) were obtained from miRbase v22.1, with the seed region in bold. MFE, minimal free energy. (b) Target sites of miR-6867-5p in the PTGR1 promoter. Sequences marked in red and blue indicate the ZFS predicted by ZHunt analysis and the seed region of miR-6867-5p, respectively. The gray-shaded box represents MER4-int. (c) HepG2 cells were transfected with miR-NC, miR-6867 mimic, or miR-6867-5p inhibitor, and the PTGR1 expression level was determined using qRT-PCR (**p < 0.01, Student’s t test). (d) HepG2 cells were co-transfected with the pGL4.11-V2 original plasmid and miR-NC, miR-6867 mimic, or miR-6867-5p inhibitor. The relative luciferase activity was expressed as the ratio of pGL4.11-V2 original (**p < 0.01, one-way ANOVA). e Relative expression of PTGR1 and miR-6867-5p in human normal sample. All data were presented as the mean ± SD. miRNA, miRNA; TSS, transcription start site; NC, negative control.
