Figure 3 | Scientific Reports

Figure 3

From: Pyromeconic acid-enriched Erigeron annuus water extract as a cosmetic ingredient for itch relief and anti-inflammatory activity

Figure 3

Anti-inflammatory and antihistamines efficacy of Erigeron annuus water extract (EEA) on PMACI-induced HMC-1 cells. (a) The MTS assay was measured. (b) The HMC-1 cells were pretreated with EEA (0.1% or 0.2%) and PA (respective concentration, 4.7 and 9.4 µg/mL) for 1 h and stimulated with 50 nM PMA + 1 µM A23187 for 24 h. Total RNA was extracted, and the mRNA expression of IL-1B, IL-6, IL-8, and IFN-r was measured by real-time qPCR. The protein expression (c) were measured using ELISA kit and (d) histamine level. Statistical comparisons were performed using a t-test (*P < 0.05. vs normal control, #P < 0.05. vs PMACI group).

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