Figure 5

Protective effects of EEA and PA on sodium dodecyl sulfate (SDS)-induced skin barrier dysfunction in HaCaT. HaCaT cells were pre-treated with EEA or PA for 6 h and stimulated 50 µg/mL SDS for 24 after pre-treatment. (a) SDS induced cytotoxicity of HaCaT cells (b) The MTT assay was investigated of EEA. (c) The MTT assay was investigated of PA (d). The mRNA level of filaggrin (FLG) and (e) caspase-14 (CASP 14) were measured by real-time qPCR (e). *P < 0.05. vs normal control, #P < 0.05. vs stimulation group.