Figure 5

Foxd1-expressing cells are absent in postnatal palatal mucoperiosteum following injury. (A) Image showing the excisional wound created in the palatal mucoperiosteum between the palatine arteries. (B) Schematic representation: Coronal view of palatal wounded tissues. The area of interest is highlighted in black dashed box (Epi oral Epithelium, PDL periodontal ligament, GT granulation tissue, A major palatine artery). (C) Anti-GFP antibody was used to detect GFP signal in the wounded tissues. Absence of signal verified that GFP (Foxd1) is not expressed in adult tissues upon injury. White arrows in merged panel indicate the leading edge of the wound. White dashed line indicates the border between the epithelium (Epi) and the granulation tissue. (D) Quantification analysis of the tdTomato-positive cells present within the wounded tissues revealed that FLP cells appear at 6 days post wounding within the granulation tissues and increase in density over time to 10 days post wounding. Quantification presented as percentage of FLP cells/field of view. (E) Quantification presented as percentage of FLP cells/total cells (tdTomato/Hoechst). Arrows point to the boundary of healthy tissue with the edge of the excisional wound. Scale bar: 100 μm.