Figure 8

FLP cells are minimally associated with p75NTR- or S100β expressing populations distal to the wound. Immunofluorescent staining for p75NTR (green) (A), day 6 and day 10 post wounding, did not reveal any significant overlap between regions of tdTomato-positive cells with p75NTR suggesting that FLP cells do not actively express this marker in repair. (B) Immunofluorescent staining for S100β (green) at day 6 and 10 post wounding. White arrows indicate the leading edge of the wound. White dashed lines indicate the border between the epithelium (Epi) and the granulation tissue (GT). White arrowheads indicate FLP cells positive for S100β marker, as indicated by the colocalization of the signal. N = 3 animals/timepoint. Scale bar: 100 μm.