Figure 1
From: MAX controls meiotic entry in sexually undifferentiated germ cells
Spatiotemporal alterations of MAX protein levels in embryonic male and female germ cells. (A) Double immunofluorescence staining of MAX and OCT4 (POU5F1) in male and female gonads at E11.5. Arrowheads indicate each OCT4-positive germ cell. (B) Gonadal sections of female germ cells at E13.5 were immunostained for MAX and SYCP3, and counterstained with DAPI. PL, preleptotene; eL, early leptotene. (C) Plots of the intensity of individual MAX staining signals in male and female germ cells and somatic cells. The relative intensities of MAX staining in individual germ and somatic cells in male and female gonads at E11.5, E12.5, E13.5, and E14.5 were determined using ImageJ software. The mean MAX signal intensity in somatic cells was arbitrarily set to 100. (D) Scatter plots representing the intensities of the MAX and SYCP3 staining signals in individual male and female germ cells at E13.5 localized in the anterior (blue dot), middle (red dot), or posterior (green dot) of the gonads. The intensities of the MAX and SYCP3 staining signals in somatic cells were set to 100.
