Figure 3
From: MAX controls meiotic entry in sexually undifferentiated germ cells
Alterations in the global expression profile of germ cells due to Max gene disruption. (A) Scatter plot of DNA microarray data from germ cells subjected to Cre-mediated Max gene knockout and their control counterparts without CreERT2 cDNA. The left and right panels show data from female and male germ cells at E12.5, respectively. Numerical values along the X- and Y-axes are the log2-transformed signal intensities of the microarray data. The upper and lower yellow diagonal lines in each panel indicate the boundaries of twofold up- and downregulation, respectively, due to Max gene disruption. Representative meiosis-related genes upregulated by Max disruption are individually marked by pink dots with gene symbols. (B) Venn diagram comparing genes activated more than twofold after Cre-mediated Max disruption in female and male germ cells at E12.5. The P-values for the significance of the overlap between two gene sets were calculated by hypergeometric tests. Meiosis-related genes included in each group are listed. (C) GO analyses of activated genes upregulated more than twofold after Cre-mediated Max disruption. Upregulated genes common to both male and female germ cells (middle panel) and those specific to either male (bottom panel) or female (upper panel) germ cells were subjected to GO analyses. Terms related to gametogenesis are indicated by red letters. (D) Venn diagram showing a significant overlap between genes activated by Max disruption in germ cells and genes designated as germline reprogramming-responsive genes50. P-values were calculated by hypergeometric tests, as in B.
