Table 2 The physicochemical prediction score for each residue of B cell epitopes from Sal k 1 allergen.

No	B cell epitope	Emini surface accessibility score (threshold = 1.000)	Parker hydrophilicity score (threshold = 1.835)	Karplus and Schulz flexibility score (threshold = 1.003)	Chou and Fashman beta turn score (threshold = 0.99)	Kolaskar and Tongaonkar antigenicity score (threshold = 1.024)
1	G	0.513	0.88	1.005	0.896	1.036
	A	1.000	1.243	0.994	0.976	1.06
	V	1.002	2.029	1.004	0.961	1.102
	K	0.931	2.1	1.019	1.026	1.101
	P	1.011	2.4	1.033	0.909	1.098
	V	2.125	2.957	1.048	0.954	1.091
	S	2.125	4.3	1.068	1.027	1.026
	E	1.36	4.3	1.081	1.106	1.018
	Q	1.511	2.686	1.084	0.973	1.045
	K	1.953	4.329	1.075	1.007	0.969
	G	1.744	3.7	1.056	1.02	0.976
	L	1.349	3.514	1.038	1.119	0.999
	E	0.501	2.129	1.026	1.05	1.052
	P	0.376	0.786	1.014	0.977	1.117
	S	0.789	0.829	0.995	0.894	1.137
5	V	1.545	4.357	1.014	1.046	1.024
	K	0.915	4.243	1.03	1.064	1.005
	P	0.953	1.386	1.035	0.993	1.008
	E	1.72	2.014	1.041	1.103	1.001
	G	1.436	3.971	1.034	1.24	0.927
	W	1.494	4.157	1.025	1.319	0.905
	S	1.387	4.857	1.037	1.324	0.864
	D	2.803	4.557	1.053	1.363	0.875
	N	4.122	4.043	1.076	1.357	0.902
	N	3.108	5.771	1.083	1.314	0.927
	K	1.88	5.143	1.068	1.204	0.934
	P	2.025	4.571	1.045	1.136	1.032
	A	2.518	4.386	1.021	1.057	1.030
	A	1.817	4.129	1.013	0.971	1.097
	Q	0.824	2.171	1.012	0.894	1.028
6	A	0.785	4.257	0.978	1.076	0.989
	A	1.553	4.557	0.992	0.994	0.962
	D	1.141	3.214	1.015	0.843	1.035
	K	1.747	3.214	1.025	0.966	1.035
	R	2.709	2.643	1.018	1.034	1.049
	V	2.342	3.086	1.003	1.077	1.027
	P	2.342	2.471	0.994	1.013	1.036
	Y	2.070	2.514	1.002	1.009	1.048
	T	2.300	0.901	1.015	0.957	1.102
	K	2.147	1.871	1.028	1.023	1.034
	Q	2.373	2.686	1.031	0.911	1.004

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