Figure 2

Phylogenetic tree of the poly(A) polymerases used in this work. The enzymes have been classified into three groups: 1°) those from eukaryotic DNA viruses, i.e. MG561 from Megavirus chiliensis, C475L from African swine fever virus, R341L from Acanthamoeba polyphaga mimivirus and VP55 from Vaccinia virus, 2°) enzymes from mouse as a representative of the animal kingdom, including the canonical poly(A) polymerase β (PAPβ, also named PAPOLB) and the non-canonical poly(A) polymerase GLD-2, 3°) PcnB poly(A) polymerase from Escherichia coli, as a representative of prokaryotes. Branch labels indicate posterior probability computed by the neighbor-joining method.