Table 1 Primer pairs and probe sequences.

From: Establishment and application of a rapid visual diagnostic method for Streptococcus agalactiae based on recombinase polymerase amplification and lateral flow strips

Primer pair

Name

Primer sequence (5′-3′)

Primer length (bp)

Product length (bp)

Set1

GBS-F1

CAGTACATACCGTCCGGGAGATCCAGGTGA

30

259

 

GBS-R1

TAAGTGAACGTGGTTATAGTGGTTGGCAGT

30

 

Set2

GBS-F2

GTTTCTCCAATGACGACATAATCTTCTGCG

30

292

 

GBS-R2

CTTGAACACTTGCCACTCTACGGGCTGCTA

30

 

Set3

GBS-F3

CGGTAAGAACTGTAGCAGCCCCTGGAGTGG

30

264

 

GBS-R3

GAGGTTGGAGCCTTGGATTTTCAGGATGTG

30

 

Set4

GBS-F4

ACGATTGTTTCGCCACTGACGACACATTCT

30

210

 

GBS-R4

TGGTGATACTGTTGTTGACTGACTGACTGA

30

 

Set5

GBS-F5

GTTTCGCCAATGATGACATATTCTTCTGCG

30

204

 

GBS-R5

TGGTGATACTGTTGTTGACTGACTGACTGA

30

 

P

 

FITC-GTTTCTCCAATGACGACATAATCTTCTGCG[THF]CAGCTTTGAAATCAA-/C3-spacer/

45

–

  1. F, R, and P represent forward primer, reverse primer, and probe, respectively. The underline represents the modified base. The bold font represents modified groups.
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