Figure 2
From: Decreased AdipoR1 signaling and its implications for obesity-induced male infertility
Expression of AdipoR1 was decreased and expression of pro-apoptotic genes, proteins and TUNEL-positive seminiferous tubules were increased in the testis from mice fed a high-fat diet. Adipor1 (a), Adipor2 (c), Bax (d) and Casp9 (e) mRNA levels, and AdipoR1 (b) and caspase-9 (f) protein levels in the testis from mice fed a normal chow diet (NC) and a high-fat diet (HF) for 22–23 weeks. Results of real-time PCR were normalized to Rn18s. Results of western blot were normalized to α-tubulin. Representative micrographs from TUNEL staining of the testis from mice fed a NC and HF (g) and percentage of seminiferous tubules with TUNEL-positive cells in mice fed a NC and HF (h). All values are presented as means ± s.e.m. *P < 0.05 and **P < 0.01 compared to mice fed a NC. P values were determined by the unpaired two-tailed t-test. Scale bar, 100 μm. The red arrows indicate TUNEL-positive cells. NS, not significant. NC, n = 15 (a, c-e), n = 5 (b, h), n = 6 (f); HF, n = 16 (a, c-e), n = 5 (b, h), n = 6 (f).
