Figure 5 | Scientific Reports

Figure 5

From: Single-cell RNA sequencing reveals critical modulators of extracellular matrix of penile cavernous cells in erectile dysfunction

Figure 5

The Disrupted Subpopulation Heterogeneity of endothelial cells Contributes to ECs alteration in ED. (A) UMAP plots showing the distributions of ECs subpopulations (ECs1–ECs12) in ED and NC corpus cavernosum samples. (B–D) The ECs subpopulation associated with endothelial cell morphogenesis was reduced in ED. (B) UMAP plot showing the distribution of the ED-associated ECs subpopulations. (C) Heatmap showing the expression levels of the top enriched genes in the ED-down-regulated ECs subpopulation (adjusted P < 0.05, log2 fold change ≥ 0.5). Down: down-regulated subpopulation; Up: up-regulated subpopulation. (D) GO pathway analysis of the transcriptomic signature of the ED-down-regulated ECs subpopulation. (E) The PPI network of the top enriched genes. (F) The developmental trajectory of ECs in each cluster inferred by Monocle2, each point corresponds to a single cell. From left to right, representing different states, different times and different groups. (G) Heatmap showing that the differentially expressed genes (rows) along the pseudo-time (columns) is clustered hierarchically into four profiles. The representative gene functions and pathways of each profile were shown.

Back to article page