Figure 8

Constructing an erectile dysfunction model in SD rats with bilateral cavernous nerve injury (BCNI) and ECM-related protein expression level expression of cavernous tissue in rats with BCNI. (A, B) The representative ICP and MAP of each experimental group were recorded under the following conditions: model: low voltage, method: continuous single stimulation, frequency: 20 Hz, intensity: 5 V. The stimulus interval indicated by the solid bar. Erectile function, as determined in vivo by electrical stimulation of the cavernous nerve of the rats in Sham group and BCNI group. (C) The erectile function of each group is presented as the maximal ICP/MAP ratio in the bar graph. (D) Masson staining of cavernous tissue sections performed Smooth muscle/Collagen ratio in the Sham group and BCNI group. (E) The ratio was quantified in the bar graph. (4 × magnification, upper row; 20 × magnification, lower row). Lower rows represent enhanced magnification of white rectangle boxes in upper rows. (F–I) Immunofluorescence staining of cavernous tissue sections performed with anti- collagen 1, anti-collagen 9 antibody in the Sham group and BCNI group (10 × whole tissue cross-sectional scan, upper row; 40 × magnification, lower row). Lower rows represent enhanced magnification of red rectangle boxes in upper rows. And staining intensity was quantified and presented as fluorescence intensity. Values are mean ± SD (n = 6 per group). #p < 0.05 compared with the BCNI group; *p < 0.05 compared with the Sham group.