Figure 3

Prepro-orexin peptides from different species. For the species codes (Supplementary Table S1), the sequences used and the alignment (Supplementary Fig. S6), see Supplementary material 1. Left, a schematic presentation of the phylogenetic tree of the taxa shown. Boxes, coding/mature regions of the peptides (both orexin-A and -B in vertebrates and a single peptide in invertebrates and early vertebrates). Black lettering indicates conserved motives, ~ denotes region of length not specified here, x denotes region with a length specified here (number of x:s' = number of aa). Colour coding as in Fig. 1. Parallel horizontal double line, signalling peptide; vertical end of the line (prepro-peptide), stop. Right, percent identity (ID) and similarity (SIM) between orexin-A and orexin-B (vertebrates), orexin-A and the sequence directly downstream that would correspond to orexin-B (tunicates, cephalocordates, echinoderms), orexin-A and “cryptic peptide” (hemichordates), or allatotropin and “cryptic peptide” (protostome) within the individual species calculated by EMBOSS Needle, BLOSUM62 global alignment. Abbreviations: aa, amino acids.