Figure 5

Binding of TAMRA-orexin-A to the plasma membrane and displacement by Ci-orexin-A(1) in CiOX-eGFP cells. Left, the cells before additions; middle, the cells after a 10 min incubation with 10 nM TAMRA-orexin-A; right, the cells after a further 10 min incubation with 1 µM Ci-orexin-A(1). Scale bar = 50 µm.