Figure 1 | Scientific Reports

Figure 1

From: Early-life influenza A (H1N1) infection independently programs brain connectivity, HPA AXIS and tissue-specific gene expression profiles

Figure 1

(A) Study outline: pups, born in-house, were subjected to early life infection (H1N1 or PBS) on post-natal day 14 (PND14). On PND42 all animals were subjected to an acute stress test in the form of restrained stress. Given ample time to recover from the stress test, mice were handled and mock-inoculated on PND56, approximately five days before being sacrificed on PND60/61. (B) Analysis of Covariance (ANCOVA) to evaluate the effect of two independent grouping variables (sex and treatment) on body weight, after adjustment for time. Estimated Marginal Means (emmean) of body weight shows significance (**** = p > 0.0001) between treatments (control = green; IAV = orange) with regards to sex. Error bars display SEM. C) Body weight development over time between different PND14 treatments for males and D) females. Boxplots display the distribution of data with the median and the inter quartile range (IQR), where individual animals are represented as points. Treatments are represented in colors (control = green; IAV = orange). P-values (*p > 0.05; **p > 0.01; ***p > 0.001; ****p > 0.0001) represent comparisons between treatments for each individual day.

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