Figure 2 | Scientific Reports

Figure 2

From: Early-life influenza A (H1N1) infection independently programs brain connectivity, HPA AXIS and tissue-specific gene expression profiles

Figure 2

Cytokine levels at PND60. We measured IL-2, IL-6, IL-10, in the blood and IL-6, TNF-α and INF-γ in the bronchoalveolar lavage fluid (BALF). The only detectable cytokines were TNF-α and IL-6 (Fig. 2A and B respectively) Overall cytokine levels were very low (< 16 pg/mL, however differences were significant for TNF-α between IAV and control. We found no sex-specificity in cytokine levels. Boxplots display the distribution of data with the median and the inter quartile range (IQR), where individual animals are represented as points. Treatments are represented in colors (control = grey; IAV = unfilled). All animals only received PBS at PND56. Animals receiving IAV at PND56 were used as controls to ensure that assays worked and non-detection of BALF cytokines was not due to experimental error.

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