Figure 3

Induced MOG-reactive CD4⁺CD25^highCD127^low regulatory T cells (Tregs) improved experimental autoimmune encephalomyelitis (EAE)-associated inflammation and demyelination in the spinal cord. (A) Histological analysis of lumber segments was performed using hematoxylin and eosin (H & E) staining. Five different regions (H & E, × 400) in each section of three sections in the lumbar segment of the spinal cord (H & E, × 40) were selected for further analysis. The wild-type (WT) group (n = 7) demonstrated no inflammatory cell infiltration, and reduced inflammatory infiltration (long red arrow) was present in the regulatory T cell (Treg)-treated group (n = 7) compared with the sham-treated group (n = 10), as shown by (B) a comparison of the scores for the degree of inflammatory cell infiltration into the spinal cords among the three groups. (C) Histological analysis of three sections in each lumber segments was performed using Luxol fast blue (LFB) staining (LFB, × 40). The wild-type (WT) group (n = 7) demonstrated no demyelination, whereas decreased demyelination (short red arrow) was present in the regulatory T cell (Treg)-treated group (n = 7) compared with the sham-treated group (n = 10), as shown by (D) a comparison of the pixel area (%) of demyelination in total white matter among the three groups. All the data are presented as the median and interquartile range.