Figure 3
From: The RoxyScan is a novel measurement of red blood cell deformability under oxidative and shear stress
A typical flow cytometry assessment of RBC exposed to oxidant and shear. (A) Dot plots showing forward scatter on the x-axis and side scatter on the y-axis in the absence of oxidant (1) and after exposure to 0.8 mM H2O2 pretreated with azide (2), CuOOH (3), or tBOOH (4). The indicated regions of intact cells (I) and fragments (II) are shown for each plot. (B) Fluorescence histograms of APC-di-annexinV labeled RBC assayed without oxidant (1) or exposed to 0.8 mM H2O2 with azide (2), CuOOH (3), or tBOOH (4).
