Figure 1
The effects of culture method on cells’ response to first-line lung adenocarcinoma therapies. (a) HCC827 cells were cultured for four days in either monolayer (2D) or hanging drop spheroid (3D) culture and challenged with erlotinib or gefitinib for 72 h. (b) The IC50 for cells in 2D culture aligns with that of the GDSC dataset while cells in 3D culture show increased resistance. (c) HCC827 cells were cultured in 2D and 3D for six days; ATP concentration was measured every 24 h. Cells in 2D culture begin proliferating earlier and show greater overall proliferation than cells in 3D culture. (d) HCC827 cells in 3D culture show increased resistance to the taxane docetaxel, but no significant difference in response to the platinum drug cisplatin. (b–d) Viability and ATP concentration were assessed via CellTiter Glo assay (Promega). (b, d). T-test with Welch’s correction; data represents the mean of three experiments; each experiment had six replicates per dose; error bars represent standard deviation. The dotted line indicates the IC50 for the indicated drug in HCC827 cells as published in the Genomics of Drug Sensitivity in Cancer (GDSC2) dataset19.
