Figure 6
From: The novel drug candidate S2/IAPinh improves survival in models of pancreatic and ovarian cancer
S2/IAPinh induces tumor cell death via activation of the extrinsic apoptosis pathway in vivo. KP2 tumor samples collected from animals at 48 h after the treatment with either vehicle, SW43, IAPinh, or S2/IAPinh, were used for analyses. (A) Representative images of TUNEL labeled apoptosis cells in KP2 tumor samples of each group. Nuclei were stained in blue with Hoechst, TUNEL positive cells are in red. Scale bars are equal to 20 µm. (B) Quantification of TUNEL positive cells per area in each group. Data are shown as means ± SEM; ****P < 0.0001. (C) Protein expression of cIAP-1, cIAP-2, and XIAP in KP2 tumor samples of each group. The precursor and cleaved forms of caspases 3, 8, and 9 were also analyzed for these cells using Wes automated capillary blotting system (Protein Simple). (D) Quantification of protein expression. Relative densitometry of each band normalized to the total protein. Data shown as means ± SEM. *P < 0.05, **P < 0.01, **** P < 0.0001.
