Table 1 Detection of facultative anaerobic and aerobic bacteria in lymphoid organs under IMO stress conditions: utilizing anaerobic culture and colony PCR, or PCR on organ homogenates in the TMA + IMO group.
Organ | Accession no | BLAST hit | Identity (%) | Oxygen usage | Gram staining | Original habitat (Ref*) |
|---|---|---|---|---|---|---|
Anaerobic culture of organ homogenates & colony PCR | ||||||
Spleen | OM_996123.1 | Escherichia coli | 100 | facultative anaerobic | negative | human feces (Méric et al., 2016) |
MT_585453.1 | Lactobacillus murinus | 100 | aerotolerant anaerobic | positive | rat feces (Yuan et al., 2020) | |
NR_125590.1 | Robertmurraya massiliosenegalensis | 100 | facultative anaerobic | positive | human feces (Ramasamy et al., 2013) | |
Mesenteric lymph node | LC_511695.1 | Staphylococcus nepalensis | 100 | facultative anaerobic | positive | oral microbiota of domestic cat (Rossi et al., 2017) |
NR_125590.1 | Robertmurraya massiliosenegalensis | 100 | facultative anaerobic | positive | human feces (Ramasamy et al., 2013) | |
NR_170510.1 | Bacillus yapensis | 100 | facultative anaerobic | positive | deep-sea sediment (Xu et al., 2020) | |
HM_439461.1 | Bacillus siralis | 100 | facultative anaerobic | positive | silage (Pettersson et al., 2000) | |
LN_995461.1 | Bacillus firmus | 100 | facultative anaerobic | positive | soil and freshwater (Huang et al., 2021) | |
Direct PCR on organ homogenates | ||||||
Spleen | MN_095260.1 | Streptococcus sp. | 100 | facultative anaerobic | positive | salivary microbiome (Kun et al., 2016) |
MN_606145.1 | Bosea vestrisii | 100 | strictly aerobic | negative | hospital water supplies (La Scola et al., 2003) | |
AB_910746.1 | Ruminococcus torques | 100 | strictly anaerobic | positive | feces of IBS patient (Kassinen et al., 2007) | |
CP_054302.1 | Acinetobacter baumannii | 98·21 | strictly aerobic | negative | hospital environment (Gootz et al., 2008) | |
KM_187436.1 | Sphingopyxis bauzanensis | 100 | strictly aerobic | negative | soil (Takeuchi et al., 2001) | |
EU_303272.1 | Methylobacterium sp. | 99·35 | facultative aerobic | negative | soil and water (Omer et al., 2004) | |
MN_746242.1 | Porphyrobacter colymbi | 96·40 | strictly aerobic | negative | freshwater pond (Fuerst et al., 1993) | |
GU_412767.1 | Actinomyces sp. | 100 | facultative anaerobic | positive | soil and microbiota of animals (Gajdács et al., 2020) | |
MN_372140.1 | Cutibacterium acens | 100 | aerotolerant anaerobic | positive | humans' skin (Kristian et al., 2022) | |
KU_991392.1 | Comamonas denitrificans | 100 | strictly aerobic | negative | activated sludge (Gumaelius et al., 2001) | |
ON_013135.1 | Staphylococcus epidermidis | 100 | facultative anaerobic | positive | human and mucosal flora (Sabaté Brescó et al., 2017) | |
Mesenteric lymph node | AB_835775.1 | Bosea sp. | 95·41 | strictly aerobic | negative | pyritic shale (La Scola et al., 2003) |
MT_322964.1 | Curvibacter gracilis | 100 | strictly aerobic | negative | soil and well water (Ding et al., 2004) | |
