Figure 4
From: Dietary linoleic acid supplementation protects against obesity-induced microglial reactivity in mice
Cortical microglial density assessed by IBA-1 immunofluorescence staining: evaluation of 5 weeks of linoleic acid (LA) supplementation on sustained high-fat diet (HFD)-induced neuroinflammation. The figure shows a significant increase in IBA-1 positive (IBA-1+) cells in obese mice (HFD-VEH) compared to non-obese animals (STD-VEH) in all cortical regions evaluated: prelimbic cortex (PL) (a), infralimbic cortex (IL) (b), orbitofrontal cortex (OFC) (c), anterior cingulate cortex (ACC) (d) and anterior insular cortex (aIC) (e). Without exception, LA supplementation for 5 weeks significantly reduced microglial density in the investigated cortical structures (a–e). Illustrative photomicrographs of ACC IBA-1+ cells for the experimental conditions STD-VEH (d1), STD-LA (d2), HFD-VEH (d3) and HFD-LA (d4), taken with a ZEISS Axio Imager Z2 microscope, equipped with ApoTome.2 and a Camera ORCA-Flash4.OLT (Zeiss, Germany), and processed to get maximal orthogonal intensity projections. Values are mean ± SEM (n = 6–8 per group). *, p < 0.05; **, p < 0.01; ***, p < 0.001.
