Figure 6
From: Dietary linoleic acid supplementation protects against obesity-induced microglial reactivity in mice
Morphometric study of microglial complexity: evaluation of 5 weeks of linoleic acid (LA) supplementation on sustained high-fat diet (HFD)-induced neuroinflammation. An increase of microglial complexity in terms of ramification (i.e. endpoints) (a) and perimeter of action (i.e. process length) (b) was found in mice under HFD (HFD-VEH) compared to control animals (STD-VEH) at hypothalamic (paraventricular nucleus—PVN), cortical (anterior cingulate cortex—ACC) and subcortical (basolateral amygdala—BLA) level. Linoleic acid supplementation reversed the microglial pathological phenotype induced by the dietary regimen (HFD-VEH vs. HFD-LA) in the regions investigated (a, b). No effect of LA supplementation on microglial morphology was observed in non-obese individuals (STD-VEH vs. STD-LA) (a, b). Illustrative 3D reconstructions of microglial cells for the experimental conditions STD-VEH (c1), STD-LA (c2), HFD-VEH (c3), and HFD-LA (c4), taken with a ZEISS Axio Imager Z2 microscope, equipped with ApoTome.2 and a Camera ORCA-Flash4.OLT (Zeiss, Germany). Values are mean ± SEM (n = 6–8 per group). *, p < 0.05; **, p < 0.01; ***, p < 0.001.
