Figure 1

Characteristics of porcine islet organoids on day 11. (A) Scheme of islet organoid formation using porcine islets. The porcine islets were cultured in advanced DMEM/F12 with Wnt-3A, R-spondin, EGF, Noggin, IGF-1, bFGF, N-acetyl-l-cysteine, [Leu¹⁵]-gastrin, and nicotinamide (organoid culture) or advanced DMEM/F12 without supplements (2D culture). (B) Islet organoids on day 11. Blue arrows indicate cystic components in the organoids. (C) Islet organoids stained with Hoechst 33342 (blue) and propidium iodide (red) to assess viability. (D) Overnight-cultured porcine islets stained with dithizone. (E) Eleven day-cultured porcine islets stained with dithizone. (F) Islet organoids on day 11 stained with dithizone (blue arrows).