Figure 6

Gene expression in Pdx1-Venus Tg porcine islet organoids on day 45. (A–G) Expression of genes correlated to carbohydrate antigens (A: Ggta1p, B: Cmah), pancreatic differentiation (C: Pdx1, G: Sox9), and endocrine hormones (D: Ins, E: Gcg, F: Sst) in overnight-cultured porcine islets (Day 1, blue) and islet organoids on day 45 (gray). The ratios of the expression compared with overnight-cultured islets are shown as 2^−ΔΔCt values. n = 3. Data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.