Figure 9
From: The porcine islet-derived organoid showed the characteristics as pancreatic duct
Transplanted islet organoids derived from microminipigs cultured for 27 days. (A) Islet organoids derived from microminipigs cultured for 27 days. They showed spheroidal and cystic components. (B) Transplanted islet organoids in the subrenal capsular space of diabetic nude mice. (C) Engrafted islet organoids on POD 28. (D) and (E) Blood glucose (D) and plasma porcine insulin (E) levels after xenotransplantation of 100 islet organoids. Graftectomies were conducted on POD 7 (n = 1), 14 (n = 1), and 28 (n = 2). (F) Immunohistochemical staining of engrafted islet organoids on POD 7 for CK19/SLA1 (green and red; upper and lower left, respectively) and porcine C-peptide (green; lower middle and right). Nuclear staining was performed using DAPI (blue). Scale bar = 200 µm (upper) and 50 µm (lower).
