Table 2 Linear mixed model analyzing associations of experimental culture conditions (predictors) and NSC passage number on monocyte-induced NSC proliferation (KI67), gliogenesis (A2B5) and neurogenesis (DCX); reported estimated 95% confidence interval (CI) and p-value.
Predictors | %_KI67 | %_A2B5 | %_DCX | ||||||
|---|---|---|---|---|---|---|---|---|---|
Estimates | CI | p | Estimates | CI | p | Estimates | CI | p | |
(Intercept) | 4.67 | 4.00 to 5.34 | < 0.001 | 5.75 | 4.94 to 6.55 | < 0.001 | 25.55 | 21.81 to 29.29 | < 0.001 |
MoC conditioning [Cond] | − 2.05 | − 2.84 to − 1.25 | < 0.001 | 2.15 | 1.31 to 2.98 | < 0.001 | 17.87 | 14.68 to 21.06 | < 0.001 |
Conditioned medium [SP-GF] | − 0.25 | − 0.82 to 0.31 | 0.372 | 0.40 | − 0.19 to 0.98 | 0.184 | 0.06 | − 2.18 to 2.31 | 0.955 |
NSC passage [Mid] | 1.23 | 0.44 to 2.03 | 0.003 | − 2.36 | − 3.19 to − 1.53 | < 0.001 | − 6.61 | − 9.77 to − 3.45 | < 0.001 |
MoC conditioning [Cond] *passage [Mid] | 0.20 | − 0.92 to 1.33 | 0.720 | − 3.36 | − 4.53 to − 2.18 | < 0.001 | − 6.55 | − 11.04 to − 2.05 | 0.005 |
Random effects | |||||||||
σ2 | 2.42 | 2.61 | 38.25 | ||||||
τ00 | 0.07 BC | 0.28 BC | 9.84 BC | ||||||
ICC | 0.03 | 0.10 | 0.20 | ||||||
N | 5 BC | 5 BC | 5 BC | ||||||
Observations | 120 | 119 | 119 | ||||||
Marginal R2/conditional R2 | 0.364/0.381 | 0.627/0.663 | 0.625/0.702 | ||||||
