Figure 6 | Scientific Reports

Figure 6

From: Mechanistic study on the alleviation of postmenopausal osteoporosis by Lactobacillus acidophilus through butyrate-mediated inhibition of osteoclast activity

Figure 6

In Vitro Validation of the Inhibitory Effects of La-02 and C4 on Osteoclast Differentiation (A) TRAcP staining to assess osteoclast differentiation; (B) Statistical analysis of TRAcP staining results; (C) RT-PCR to measure the expression levels of osteoclast-related genes CTSK, Acp5, and c-Fos; (D) IF staining for the expression of osteoclast-related proteins; (E) Statistical analysis of IF staining results; (F) Assay kit to evaluate osteoclast bone resorption ability; (G) WB to measure RANK expression, with β-actin as the internal reference protein. * represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001, **** represents P < 0.0001.

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