Figure 1
Susceptibility of Col-0 and Mediator mutants to infection by P. syringae. Four leaves each of 5-week-old plants were injected with either 10 mM MgCl2 (control) or 1 × 106 CFU/ml of the virulent Pst. DC3000. (A) Bacteria were extracted from 100 mg of grounded tissue from mock-treated and infected plants and colonies formed from 10 × serial dilutions (D1 to D6) at the 72-h time point are shown. (B) The box plot represents the means of CFUs of the D3 dilution from four independent infections. Asterisks indicate significant differences between each mutant and Col-0 (Student’s t test, *p < 0.05; **p < 0.01; ***p < 0.001) and the error bars show mean standard deviation (SD) of four independently infected plants. (C) An illustrative picture of disease symptoms visible as necrotic and chlorotic lesions on leaf surfaces from three independently infected plants (infection 1–3) and one control experiment.
