Figure 4
Impaired reduction of specific metabolite levels is caused by different mechanisms in med16 and med18. (A) Heat map and hierarchical clustering of metabolites that show a log2-FC > 0.5 or < − 0.5, and p < 0.05 in infected relative to mock-treated Col-0 at the 24-h time point. (B) Bar graphs of normalized mean peak areas from four independently infected or mock-treated Col-0 and mutant plants at the 24-h time point. (C) Quantification of transcript levels for two of the aliphatic-GSL biosynthesis enzymes (CYP79F1 and MAM1) in uninfected Col-0 and mutants using RT-qPCR. (D) Quantification of transcript levels for two of the aliphatic-GSL biosynthesis regulating transcription factors (MYB29 and MYB76) in uninfected Col-0 (black bars) and mutants (light green bars) using RT-qPCR. Asterisks in B-D indicate pairwise significant differences (Student's t test, *p < 0.05; **p < 0.01; ***p < 0.001) for comparisons indicated by the brackets. Error bars represent the SD of four independent infection replicates for metabolite levels and three replicates for transcript levels. (E) Simplified overview of the aliphatic-GLS, indolic-GLS and phytoalexin synthetic pathways. Each arrow indicates multiple enzymatic steps and only the enzymes analyzed here are indicated.
