Figure 6
med25 shows an attenuated infection response whereas med16 and cdk8 show the highest alterations in metabolite levels compared to Col-0 at the late stage of infection. (A) Scatter plots of the relationship between metabolite changes at 72 h p.i. (relative to control at 72 h) between each mutant (x-axis) and Col-0 (y-axis). (B) Upset diagram illustrating the overlap and uniqueness in increased and decreased metabolite levels in each mutant relative to Col-0 at 72 h p.i. Total number of metabolites displaying changed levels in each mutant are represented as red (increased) and blue (decreased) bars to the left. (C) Distribution of metabolites displaying changed levels in each mutant for each of the nineteen metabolite categories. Sizes of circles represent the ratio of the of the number of metabolites in each category that display significantly distinct levels in mutants relative to Col-0 and the total amount of metabolites detected for each category in our full data set of 222 metabolites. Numbers to the right of each circle represent the total number of metabolites that show significantly changed levels in each mutant. In (A) a significance cutoff of log2-FC > 0.5 or < − 0.5 and p < 0.05) was used. In (B, C) a significance cutoff of log2-FC > 1 or < − 1 and p < 0.05) was used. The experiments were performed using four independently infected plants. (D) Levels of two JA activated genes (MYC2 and JMT) in mock-treated and infected cells at the 24-h time point. Asterisks indicate pairwise significant differences between mock-treated and infected lines as indicated by the brackets (Student's t test, *p < 0.05; **p < 0.01; ***p < 0.001) and the error bars show the SD of three independently infected plants.
