Figure 2
Images of 200 nm beads through water captured by mosTF and lineTF. The objective's working distance is 2 mm. The full FOV is 205 × 205 µm2. Images (a, b, f, g) are displayed with the same color scale [0–300]. (a) mosTF image of the full FOV. (b) lineTF image of the full FOV. (c) Intensity distribution of beads in (a, red) and (b, blue). (d) SBR comparison (n = 619). The median SBR of mosTF is 156.66, and that of lineTF is 47.62. (e) MSE comparison. The mosTF image serves as a reference, resulting in an MSE of zero for mosTF. The MSE of lineTF is 1.23. (f, g) Zoomed-in views of the area marked by a green box in (a) and (b), respectively. (h) Intensity profile along the cross-section of the two beads in (f) and (g), indicated by the green dashed line. (i) PSF profile along the x- (blue), y- (red), and diagonal (green) directions (n = 295). Full Width at Half Maximum (FWHM) values: FWHMx: 1.15 ± 0.26 µm; FWHMy: 1.15 ± 0.26 µm; FWHMdiag: 1.00 ± 0.24 µm. Scale bar in (a, b), 50 µm. Scale bar in (f, g), 5 µm.
