Figure 13

Cytogram of cellular apoptosis against MDA-MB-231 cells induced by free DTX and DTX-loaded NPs in their IC50 dose in comparison to the blank hyperbranched MeO-PEG-b-(NIPAAm-co-PBAE) copolymeric NPs as positive control and non-treated cells as a negative control. The flow cytometric diagram divided cells into four sections (viable, early apoptotic, late apoptotic, and necrotic) and the percent of counted cells in each area is illustrated on the quadrant charts (Pvalue < 0.001) (A). Quantitative results of apoptotic effects evaluated by Annexin V/FITC assay (B).