Figure 2
Prevalence of trench- and pit-formation events in the absence or presence of an ectosteric (T06) or active site-directed (ODN) CatK inhibitor over a period of 72 h (time-lapse). (A,B) The proportion of OCs in trench mode as a total percentage, and proportion of trench-making OCs stopping their elongation. (C,D) The proportion of resorption events starting either as a pit or directly as a trench. (E–G) The proportion of OCs in pit mode either starting as single or multiple pits. All are shown as total percentage. These proportions were assessed in independent experiments. Sample size: Control: n = 16 from 5 donors; 200 nM T06: n = 12 (3 donors); 300 nM T06: n = 12 (3 donors); 500 nM T06: n = 13 (4 donors); 1 µM T06: n = 7 (2 donors); 15 nM ODN: n = 12 (3 donors); 50 nM ODN: n = 7 (2 donors). For each donor, 3 to 5 replicate experiments on individual bone slices were analyzed for all conditions. We analyzed between 50 and 150 OCs per condition per experiment for each of the donors. The median proportions obtained in each experiment are shown as bars. Statistics: Kruskal–Wallis test, two tailed, Dunn’s multiple comparisons test compared groups with control verified significance difference. The * indicated in these graphs are based on the values obtained as per Kruskal; Dunn’s tests and summarized accordingly and represented as (ns, Not significant; *P < 0.05; **P < 0.01; ***P < 0.0001).
