Figure 2
From: Technical considerations for cost-effective transposon directed insertion-site sequencing (TraDIS)
Higher quantity of transposon DNA during transposome assembly improves electroporation efficiency. We assembled the transposome by mixing 1U of Tn5 transposase with various amounts of the KAN2 transposon (100, 200, 400 ng). We electroporated our E. coli cohort and measured CFUs the following day. Bars indicate normalized CFUs as a function of transposon quantity during assembly. Error bars indicate variance among replicates (p = 7.95e–12). Bars with red highlights indicate the recommended transposon quantity in a standard TraDIS protocol (EZ-Tn5 Transposase manual).
