Figure 4
From: Technical considerations for cost-effective transposon directed insertion-site sequencing (TraDIS)
Altering recovery time after electroporation does not significantly affect the number of unique mutants: We electroporated our Escherichia coli cohort with 0.05U of Tn5 transposome and recovered for 0.5, 1 or 2 h before plating. We determined CFUs on the following day. (A) Average CFUs among the E. coli cohort at respective time points, which are normalized to CFUs at 1 h recovery time. FC fold change. (B) The number of unique insertion sites for each E. coli strain at respective time points (p = 0.4).
