Figure 3
From: Key HPI axis receptors facilitate light adaptive behavior in larval zebrafish
Illumination and genotype are determinants of basal locomotion in nr3c1 knockout larvae. Aa Ba Locomotor activity (mean predicted value [mm/min] ± 95%CI) for each genotype and experimental condition ((A) in darkness, (B) in light). (Ab, Bb) Basal locomotor activity of nr3c1 knockout larvae over 12 h. The scatterplot (points) shows actual mean locomotor activity (mm/min) for each experimental condition of each assay. The line graph shows predicted locomotor activity for each experimental condition by the GAM (predicted value ± 95%CI). (Ac, Bc) Time points where an experimental condition showed significantly higher locomotor activity compared to the other in a pairwise comparison. The color shown indicates the group with a significantly higher outcome in a pairwise comparison. (Ad, Bd) Histogram of individual fish locomotion (y-axis is the same as subpanel [b]). Density distribution of actual mean locomotor activity shows severely right skewed distribution (low locomotor response). The integration of the curve equals 100%. (D: dark, L: light, WT: wildtype, HT: heterozygous, HM: homozygous, n.s: not significant, nr3c1 genotypes: nr3c1+/+ [WT], nr3c1+/mn63 [HT], nr3c1mn63/mn63 [HM, KO], or nr3c1+/mn65 [HT], nr3c1mn65/mn65 [HM, KO]).
