Figure 4
From: Key HPI axis receptors facilitate light adaptive behavior in larval zebrafish
mc2r knockout larvae respond differentially in darkness based on the durations of illumination and genotype. (Aa, Ba, Ca) Locomotor activity (mean predicted value [mm/min] ± 95% CI) for each experimental condition predicted by the GAM for each photo period (gray: dark, white: light period). (Ab, Bb, Cb) Locomotor response of mc2rex1 larvae during dark–light repeat assays. The scatterplot (points) shows actual mean locomotor activity (mm/min) for each experimental condition of each assay. The line graph shows predicted locomotor activity for each experimental condition by the GAM (predicted value ± 95% CI; gray: dark, white: light period). (Ac, Bc, Cc) Time points where an experimental condition showed significantly high locomotor activity compared to the other in a pairwise comparison. The color shown indicates the group with a significantly higher outcome in a pairwise comparison. (Ad, Bd, Cd) Histogram of individual fish locomotion (y-axis is the same as subpanel [b]). Actual mean locomotor activity shows severely right skewed distribution (low locomotor response). The integration of the curve equals 100%. (D: dark, L: light, WT: wildtype, HT: heterozygous, HM: homozygous, n.s: not significant, mc2r genotypes: mc2r+/+ [WT], mc2r+/mn57 [HT], mc2rmn57/mn57 [HM, KO], mc2r+/mn58 [HT], mc2rmn58/mn58 [HM, KO], or mc2r+/mn59 [HT], mc2rmn59/mn59 [HM, KO]).
