Figure 7
From: Key HPI axis receptors facilitate light adaptive behavior in larval zebrafish
nr3c1 (gr) and nr3c2 (mr) double knockout larvae show varying locomotor activity levels in darkness after short illumination (1-min) (a) Locomotor activity (mean predicted value [mm/min] ± 95% CI) for each genotype (gr WT mr WT, gr WT mr HM, gr HM mr WT, gr HM mr HM), predicted by the GAM. Only WT and HM mutants are shown to increase readability in this figure while the full genotypic combinations are shown in Supplementary Fig. S87. The underlying data set is the same in the two figures. (b) Locomotor response in the baseline (dark) and post-illumination (dark). The scatterplot (points) shows actual mean locomotor activity (mm/min) for each experimental condition of each assay. The line graph shows predicted locomotor activity for each genotype by the GAM (predicted value ± 95% CI). (c) Time points where a genotype showed significantly high locomotor activity compared to the other in a pairwise comparison. (d) Density distribution of actual mean locomotor activity shows severely right skewed distribution. The integration of the curve equals 100% (gr: glucocorticoid receptor [nr3c1], mr: mineralocorticoid receptor [nr3c2], D: dark, T: treatment [illumination], WT: wildtype, HM: homozygous, n.s: not significant, nr3c1 genotypes: nr3c1+/+ [WT], nr3c1+/mn63 [HT], nr3c1mn63/mn63 [HM, KO], or nr3c1+/mn65 [HT], nr3c1mn65/mn65 [HM, KO], nr3c2 genotypes: nr3c2+/+ [WT], nr3c2+/mn67 [HT], nr3c2mn67/mn67 [HM, KO]).
