Figure 4
Determination the effects of PHB1 on DENV infection in HEK293T/17 cells. HEK293T/17 cells were mock transfected or transfected with 2 µg of a plasmid expressing PHB1 or an empty vector. At 24 h post-transfection cells were either mock-infected or infected with DENV 2 or DENV 4 at MOI 5 for 72 h. Subsequently proteins were extracted and used in (A) western blot analysis to detect expression of DENV E protein, DENV NS3 protein, PHB1 and actin. Protein bands were quantitated for (B) DENV 2 E protein, (C) DENV 2 NS3 protein and (D) DENV 4 E protein. In parallel (E) percentage of infection (F) virus titer and (G) cellular viral genome copy number were determined. All experiments were undertaken independently in triplicate. Error bars represent S.E.M. p value ** < 0.01 and * < 0.05. Full, uncropped western blots can be found in the supplemental materials.
