Figure 2
Optimization of AP models. (a) Five parameters characterizing AP: maximum diastolic potential (MDP), maximum upstroke velocity (Vmax), AP duration of 90% repolarization (APD90), APD90/50, and beat frequency (BF). (b) APs calculated from the optimized models for ten independent GAs are overlaid. The solid black line indicates the best model and the solid gray line indicates the other nine. All APs are shown to be aligned with the rise timing as 0 ms. (c) Comparison of the AP parameters. Experimental values of mouse iPS-CMs, values calculated using the proposed model, and values calculated using the original Paci model. (d) Optimized conductances are shown as scaling factors for the base model. We plotted the scaling factors of the optimized models for the ten independent GAs. The numbers in this panel are the conductances of the best model. Na: fast sodium current, CaL: L-type calcium current, to: transient outward current, Kr: rapid delayed rectifier current, K1: inward rectifier current, f: hyperpolarization-activated cyclic-nucleotide-gated funny current, NaCa: sodium-calcium exchanger, and NaK: sodium–potassium pump. (e) Overlay plot of AP waveforms calculated using the original Paci model (blue dashed line), optimized Paci model after replacing If with that of the mouse embryonic cardiomyocyte model (black solid line), and optimized Paci model (red dotted line). (f) Pacemaker-like cell AP model optimized to reproduce the experimental results. The APs calculated from the optimized models for the ten independent GAs are overlaid. The solid black line indicates the best model and the solid gray line indicates the other nine. All APs are shown to be aligned with MDP as 0 ms.
