Figure 1

Hem1-deficiency leads to osteopetrosis-like phenotype. (A) Hem1^-/- mice display signs of growth retardation and reduced femoral length. (B) Representative µCT cross sections of distal femora of WT and Hem1^-/- animals. (C) Statistical analysis of trabecular bone volume to tissue volume (BV/TV,) trabecular thickness (Tb.Th.), trabecular number (Tb.N.) and trabecular separation (Tb.Sp.) of trabecular bone from 8–12 weeks old WT and Hem1^-/- male mice. **** P < 0.0001 (BV/TV, Tb.Th.), ** = P 0.0062 (Tb.N.), *** P = 0.0005 (Tb.Sp.), two-tailed Mann–Whitney U test. (D) Representative H&E staining of distal femora from WT and Hem1^-/- mice, red box represents magnified area. (E) Left: Representative TRAP staining of distal femora from WT and Hem1^-/- mice, red box represents magnified area. Right: Quantification of TRAP-positive osteoclasts within trabecular region from distal femora of WT and Hem1^-/- mice. * P = 0.0317, two-tailed Mann–Whitney U test. All data presented as mean ± SEM.