Figure 1 | Scientific Reports

Figure 1

From: Label-free quantification of imaging features in the extracellular matrix of left and right-sided colon cancer tissues

Figure 1

Sample origin and processing workflow. (A) Schematic presentation of the sites from which LSCC and RSCC samples were obtained. (B) Schematic of the optical setup. The excitation wavelength of 870 nm illuminates the tissue and the photons are scattered in the forward and back direction. The condenser collects the forward scattered photons and the light passes through a 434/20 nm filter to collect F-SHG signal by the PMT. The light path of the backscattered photons is further split by 495 nm and 525 nm long pass filters. The 2PEF and B-SHG are collected at the PMTs after light passes through 525/50 nm and 434/20 nm bandpass filters, respectively. (C) Pre-processing of samples, as well as, sectioning and imaging protocol. Created with BioRender.com.

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