Figure 5

Differences in the imaging features of collagen fibres obtained by texture analysis on the F-SHG signal from 2PLSM images. (A) Collagen fibres in tumour regions are straighter compared to the healthy tissues. (B) The waviness of the fibres does not change in left and right sided colon cancer. (C) Coherence cannot segregate the tumour from healthy tissues. (D) Coherence can distinguish the LSCC, which has a higher degree of organisation than the RSCC. (E) The percentage of regions of low organisations in tumours increases significantly whereas healthy tissues have uniform distribution of organised and unorganised structures. (F) The low coherence (chaotic) regions in RSCC are higher than LSCC. Orange points indicate ROIs from a CRC derived metastasis and yellow points indicate ROIs from MSI tumour samples. Data is presented as mean ± SD, ** indicates p ≤ 0.01, *** indicates p ≤ 0.001, **** indicates p ≤ 0.0001; ns = not significant.