Table 1 Strains and plasmids used in the study.
From: Phylogeny and structural modeling of the transcription factor CsqR (YihW) from Escherichia coli
Strain | Description | References |
|---|---|---|
K-12 MG1655 | Wild type F- lambda- ilvG- rfb-50 rph-1 | |
K-12 MG1655_yihW_his | K-12 MG1655 with 6xhis-tag added to the 3’-end of the csqR (yihW) gene | This work |
BL21*(DE3) | F- ompT hsdSB (rB-mB-) gal dcm rne131 (DE3) | |
BL21-CodonPlus(DE3)-RIL | F- ompT hsdSB (rB- mB-) gal dcm (DE3) endA Hte [argU proLCamr] [argU ileY leuW Strep/SpecR ] | |
OverExpress C41(DE3) | F- ompT hsdSB (rB- mB-) gal dcm (DE3) | |
Plasmids | ||
pGEM ∆Xba | Expression vector based on pGEMEX1 (Promega) AmpR | |
pGEMEX_yihW_TEV_his | Vector overproducing C-6x-his-tagged CsqR (YihW) protein, AmpR | This work |
