Table 4 Box-Behnken design portfolio and results.
Serial number | Substrate concentration (C) | Inoculum volume (mL) | Temperature (°C) | CMCase activity (U/mL) |
|---|---|---|---|---|
1 | 1 | 2 | 37 | 4.63 |
2 | 3 | 2 | 37 | 6.53 |
3 | 1 | 8 | 37 | 5.03 |
4 | 3 | 8 | 37 | 4.69 |
5 | 1 | 5 | 30 | 4.66 |
6 | 3 | 5 | 30 | 12.04 |
7 | 1 | 5 | 44 | 3.14 |
8 | 3 | 5 | 44 | 6.79 |
9 | 2 | 2 | 30 | 5.22 |
10 | 2 | 8 | 30 | 3.89 |
11 | 2 | 2 | 44 | 2.66 |
12 | 2 | 8 | 44 | 3.82 |
13 | 2 | 5 | 37 | 14.71 |
14 | 2 | 5 | 37 | 17.63 |
15 | 2 | 5 | 37 | 16.17 |
16 | 2 | 5 | 37 | 15.42 |
17 | 2 | 5 | 37 | 16.92 |