Figure 3

Beneficial impact of CA supplementation on BPA-induced change in pancreatic islet cell morphology. Representative photomicrograph of haematoxylene-eosine stained pancreas sections of BPA (10 and 100 µg/kg body weight for 21 days)-treated mice with or without supplementation of ethanol extract of Centella asiatica (CA: 200 and 400 mg/kg body weight/day for 21 days). (a) Control, (b) CA supplemented (200 mg/kg body weight/day for 21 days), (c) CA supplemented (400 mg/kg body weight/day for 21 days), (d) BPA treated (10 µg/kg body weight for 21 days), (e) BPA treated (100 µg/kg body weight for 21 days), (f) BPA10 + CA supplemented (200 mg/kg body weight/day for 21 days), (g) BPA10 + CA supplemented (400 mg/kg body weight/day for 21 days), (h) BPA100 + CA supplemented (200 mg/kg body weight/day for 21 days) and (i) BPA 100 + CA supplemented (400 mg/kg body weight/day for 21 days). Magnification ×400 and scale bar: 50 µm for all panels. (j) Quantification of cytoplasmic vacuolization of pancreatic islets. Vacuolization score was calculated using the following: > 2 vacuoles/100 μm islet diameter—1, > 4 vacuoles/100 μm islet diameter—2, > 8 vacuoles/100 μm islet diameter—3 (n = 6; from each animal, 3 islets were considered for scoring). (k) Median islet diameter (µm). Data of median islet diameter were presented as mean ± SEM (n = 6). Normality of data was tested by Shapiro–Wilk test. Significance level based on one way ANOVA, P < 0.001. Significance level based on Tukey’s post hoc test *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant.