Table 1 RNA extraction following blood transfer from EDTA to Paxgene tubes.

From: Extraction of high quality and high yield RNA from frozen EDTA blood

Protocol

Yield (μg RNA/ml blood)

% PP-Ctrl

RIN

260/280

260/230

Paxgene Ctrl

PreAnalytix

1.00 ± 0.31

100.00

8.00 ± 0.2

2.14 ± 0.06

1.54 ± 0.04

EDTA blood transfer to Paxgene tube

Kit

PreAnalytix

1.38 ± 0.48

136.76 ± 5.74*

4.00 ± 0.3**

2.15 ± 0.08

0.94 ± 0.11*

Magmax

1.04 ± 0.23

107.10 ± 9.56

4.55 ± 0.25**

2.15 ± 0.05

0.39 ± 0.14**

Incubation

2 h

0.85 ± 0.03

123.07 ± 3.65

4.87 ± 0.30**

2.08 ± 0.01

0.59 ± 0.21**

overnight

1.37 ± 0.22

104.83 ± 17.31

3.97 ± 0.15**

2.22 ± 0.01

0.57 ± 0.11**

Refreeze

No

1.21 ± 0.24

107.27 ± 11.98

4.28 ± 0.23**

2.15 ± 0.04

0.66 ± 0.17**

Yes

0.90 ± 0.05

83.60 ± 6.47

4.70 ± 0.57**

2.16 ± 0.06

0.42 ± 0.03**

  1. EDTA blood was thawed on ice for at least 2 h before transferring to Paxgene tubes. Data are expressed as Mean ± SEM. N = 2.
  2. *P < 0.05, **P < 0.01.
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